Elderberry (Sambucus Nigra) Bark Contains two Structurally Different Neusac(alpha2,6)Gal/Galnac-Binding Type 2 Ribosome-Inactivating Proteins
نویسندگان
چکیده
منابع مشابه
Sambucus nigra (Elderberry) Monograph
Introduction Sambucus nigra, or European elder, is a tall tree-like shrub, native to Europe, Asia, and North Africa, and naturalized in the United States. Various parts of the elder have long been used in traditional medicine as a diaphoretic, diuretic, astringent, laxative, and emetic. The berries were used traditionally as a food to make elderberry wine and pies, and as a flavoring or dye. Cu...
متن کاملMutational analysis of the carbohydrate-binding activity of the NeuAc(alpha-2,6)Gal/GalNAc-specific type 2 ribosome-inactivating protein from elderberry (Sambucus nigra) fruits.
Sambucus nigra agglutinin I (SNA-I) is a type 2 ribosome-inactivating protein. Site-directed mutagenesis was used to mimic the conversion of the highly active B-chain of fruit-specific SNA (SNA-If) into the completely inactive B-chain of the closely related and naturally occurring loss-of-activity mutant called S. nigra agglutinin lectin-related protein. In the first mutant SNA-If-M1 the high-a...
متن کاملFractionation of sialylated oligosaccharides, glycopeptides, and glycoproteins on immobilized elderberry (Sambucus nigra L.) bark lectin.
A new plant lectin from elderberry (Sambucus nigra L.) bark, which was shown by immunochemical techniques to bind specifically to terminal Neu5Ac(alpha 2-6)Gal/GalNAc residues of glycoconjugates, was immobilized onto Sepharose 4B (SNA-Sepharose) and its carbohydrate binding properties was determined using a series of standard compounds. Oligosaccharides, glycopeptides, or glycoproteins containi...
متن کاملIsolation and characterization of a second lectin (SNA-II) present in elderberry (Sambucus nigra L.) bark.
A second lectin (SNA-II) has been isolated from elderberry (Sambucus nigra L.) bark by affinity chromatography on immobilized asialo-glycophorin. This lectin is a blood group nonspecific glycoprotein containing 7.8% carbohydrate and which is rich in asparagine/aspartic acid, glutamine/glutamic acid, glycine, valine, and leucine. Gel filtration on Superose 12 gave a single symmetrical peak corre...
متن کاملAnthocyanins and other polyphenolics in American elderberry (Sambucus canadensis) and European elderberry (S. nigra) cultivars.
BACKGROUND Ten genotypes representing two elderberry species, Sambucus canadensis L. (eight genotypes) and S. nigra L. (two genotypes), were examined for their anthocyanins (ACY), total phenolics (TP),°Brix, titratable acidity (TA), and pH over two growing seasons. RESULTS Overall, fruit generally had higher ACY, TP, ACY/TP,°Brix, and pH in 2005 than 2004. All samples of S. canadensis had sim...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1997
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1997.00648.x